Journal: BMC Immunology

Article Title: Changes of B cell subsets in central pathological process of autoimmune encephalomyelitis in mice

doi: 10.1186/s12865-019-0301-4

Figure Lengend Snippet: Changes in B cell subsets in EAE mice over 30 day experimental period. The activated B-cells, memory B-cells and plasma cells were determined using markers CD19 + and IgD + (activated B-cell), CD19 + , CD27 + and B220 + (memory B-cell) and CD19 − and CD138 + (plasma cell).* denotes P < 0.05 vs control

Article Snippet: The primary antibodies were antibodies against rabbit IL-4 (bs-0581R, Bioss), IL-17 (bs -1183R), IL2RA/CD25 (bs-0577R), IFN-gamma (bs-0480R), syndecan-1 (bs-1309R), CD27 (bs-2491R), CD19 (bs-4755R), CD4 (bs-0647R) and CD45 (bs-10599R), polyclonal antibody against CD3E (A1753, ABdonal) and plasma cell antibody[LIV3G11] (ab44876, abcam).

Techniques:

Journal: BMC Immunology

Article Title: Changes of B cell subsets in central pathological process of autoimmune encephalomyelitis in mice

doi: 10.1186/s12865-019-0301-4

Figure Lengend Snippet: Immunohistochemical assays ( a ) and relative expression ( b ) of CD19 + cells in the spinal cord and brain tissue of EAE mice (× 200). A and G. control at day 7; B and H, EAE mice at day 7; C and I, control at day 18; D and J, EAE mice at day 18, E and K. control at day 30 and F and L. EAE mice at day 30, respectively. A-F, spinal cord tissue; G-L, brain tissue. Arrows denote CD19 + cells. * denotes P < 0.05 vs control

Article Snippet: The primary antibodies were antibodies against rabbit IL-4 (bs-0581R, Bioss), IL-17 (bs -1183R), IL2RA/CD25 (bs-0577R), IFN-gamma (bs-0480R), syndecan-1 (bs-1309R), CD27 (bs-2491R), CD19 (bs-4755R), CD4 (bs-0647R) and CD45 (bs-10599R), polyclonal antibody against CD3E (A1753, ABdonal) and plasma cell antibody[LIV3G11] (ab44876, abcam).

Techniques: Immunohistochemical staining, Expressing, Mouse Assay

Journal: Cell Reports Medicine

Article Title: Virus envelope glycoprotein targeting bispecific T cell engager protects mice from lethal severe fever with thrombocytopenia virus infection

doi: 10.1016/j.xcrm.2025.102458

Figure Lengend Snippet: BiTEs provided treatment effects against lethal SFTSV infection through improving T cell cytotoxicity in vivo (A) Percentage of CD45 + T cells, CD4 + T cells, CD8 + T cells, CD19 + B cells, CD11b + Gr-1 + neutrophils, and Foxp3 + Tregs in spleen samples collected at 6 dpi from mice ( n = 5 per group) measured by flow cytometry. (B) A schematic diagram showing depletion of T cells in mice to determine the role of T cells in 3A5 BiTE treatment. Six- to eight-week-old male humanized CD3e C57BL/6 mice were pretreated with anti-IFNAR1 antibody (300 μg per mouse) and then intraperitoneally inoculated with SFTSV (2 × 10 4 PFUs per mouse) one day later. One day after viral infection, mice were intraperitoneally injected with 3A5 BiTE (10 mg/kg for each mouse) or an equal volume of PBS. Anti-mouse CD4 and CD8a antibodies were used for depletion of T cells. (C) Survival curves of SFTSV-challenged mice treated with PBS ( n = 12) or 3A5 BiTE ( n = 12) and mice treated with 3A5 BiTE and depleted with CD4 + or CD8 + T cells ( n = 7). Death is defined as a humane endpoint when animals lost ≥20% body weight but not natural death. (D) Viral titers in spleen samples from mice measured by immunological focus assay, at 6 days post-infection (dpi). (E) Representative images of spleen sections collected at 6 dpi stained with hematoxylin and eosin (H&E) or with an antibody against SFTSV NP. Scale bars, 100 μm. See also . Data were presented as mean ± SD (A and D). One-way ANOVA followed by Tukey’s multiple comparisons test was performed for comparison of continuous variables among multiple groups (A and D). The Kaplan-Meier method was used to analyze time-to-event data (C). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns: no significance.

Article Snippet: CD19 polyclonal antibody , Signalway Antibody , Cat# 41946 RRID: AB_2933698.

Techniques: Infection, In Vivo, Flow Cytometry, Injection, Staining, Comparison

Journal: Cell Reports Medicine

Article Title: Virus envelope glycoprotein targeting bispecific T cell engager protects mice from lethal severe fever with thrombocytopenia virus infection

doi: 10.1016/j.xcrm.2025.102458

Figure Lengend Snippet: scRNA-seq analysis of splenocytes from mice treated with 3A5 BiTE (A) Immune cells of the spleen from SFTSV-infected mice following treatment with control BiTE or 3A5 BiTE were isolated for scRNA-seq analysis ( n = 3 per group). The UMAP plot displays all the immune cells from the two groups, colored by eight major cell types. (B) Proportion of the eight major cell types in the control BiTE group and 3A5 BiTE group. (C) Violin plots show cytotoxicity (left) and exhaustion (right) module score for CD4 + T ( n = 936 and n = 532) and CD8 + T ( n = 1997 and n = 1174) cells in the 3A5 BiTE-treated group and the control group, respectively. The horizontal lines display the 25 th , 50 th (median), and 75 th percentiles. The cytotoxicity module score was calculated based on the expression levels of Gzma, Gzmb, Gzmk, Gzmc, Gzmm, Gzmf, Ifng, Nkg7, Prf1, Ccl4, and Ccl5. The exhaustion module score was calculated based on the expression levels of Pdcd1, Lag3, Ctla4, Havcr2, and Tnfrsf9. A two-tailed t test was performed to analyze the differences between two groups. ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; ns: no significance. (D) The UMAP plot displays T cells colored by 10 T cell subsets. (E) The bubble plot shows expression of well-documented marker genes in T cell subsets. Dot size represents gene expression percentage per cell type, with marker gene intensity displayed. (F) Comparison of the proportions of T cell subsets between control BiTE group and 3A5 BiTE group. Data were presented as mean ± SD Mann-Whitney test was used for comparisons of values among two groups. ∗ p < 0.05; ∗∗ p < 0.01; ns: no significance. (G) Heatmap of GSEA pathway enrichment scores of individual T cell clusters between 3A5 BiTE and control BiTE groups. (H) A circle plot showing differential interaction strength among CD4 + T cells, CD8 + T cells, B cells, and plasma cells across two groups. Red edges indicate stronger interactions in the 3A5 BiTE group in comparison with control BiTE group, while blue edges indicate stronger interactions in the control BiTE group compared with 3A5 BiTE group. (I) Spleen sections collected from 3A5 BiTE- and control BiTE-treated mice at 6 days post-infection were subjected to co-immunofluorescence staining using anti-CD4 and anti-CD19 antibodies. Scale bars, 100 μm. See also and .

Article Snippet: CD19 polyclonal antibody , Signalway Antibody , Cat# 41946 RRID: AB_2933698.

Techniques: Infection, Control, Isolation, Expressing, Two Tailed Test, Marker, Gene Expression, Comparison, MANN-WHITNEY, Clinical Proteomics, Immunofluorescence, Staining